Gene Manipulation Q & A – Applications of Gene Manipulation

1. How many routes are available for the biosynthesis of nucleotides in human beings?
a) 1
b) 2
c) 3
d) 4

2. What are the basic precursors in the de novo pathway of nucleotide synthesis?
a) Carbohydrate, proteins
b) Sugars, amino acids
c) Sugars, vitamins
d) Minerals, carbohydrates

3. In the salvage pathway, nucleotides are synthesized by the recycling of __________
a) DNA
b) RNA
c) DNA, RNA
d) Sugars

4. De novo pathway is exploited for the selection of cells carrying functional HPRT and TK genes.
a) True
b) False

5. The drug aminopterin blocks the _________ of two enzymes.
a) Salvage pathway
b) De novo synthesis
c) Expression
d) Recombination

6. What do you understand by the term “co-transformation”?
a) Integration of 2 transgenes
b) Integration of similar transgenes
c) Integration of a group of transgenes
d) Integration of chromosomal DNA

7. Southern blot hybridization is done for testing the presence of _________ in selection of transformants.
a) Non-selected DNA
b) Selected plasmid DNA
c) Genomic DNA
d) Selected genomic DNA

8. Incorporation of ribozymes into antisense RNA leads to their __________
a) Activity
b) Temperature change
c) Cleavage
d) Regeneration